ABSTRACT
Objective: To investigate the distribution feature of TCR D~X gene rearrangement in peripheral blood T cells and thymocytes from healthy individuals. Methods: The rearrangement segments of TCR D?X gene with J?X, D?3 or Ja were amplified in genomic DNA from peripheral blood mononuclear cells(PBMCs) of 10 cases, sorted CD3 + T cells from peripheral blood of 4 cases and thymocytes from 7 cases, by using semi-nested PCR. Different amounts of DNA from all samples were amplified to estimate the frequency of D?X gene rearrangements. Results: The rearrangements of TCR D?X with J?X,D?3 or PJa respectively could be found in the most samples of peripheral blood or thymocytes.The frequency of D?X rearrangements were different in peripheral blood cells and thymocytes by analysis of PCR with different amounts DNA. Conclusion: The TCR D?X, Ja was the most frequent rearrangement in mature and immature T cells, whereas TCR D?X,D?3 were more frequently rearranged in immature T cells.